细粉碎小麦诱导亚急性瘤胃酸中毒对奶牛乳脂合成和肝脂肪合成关键酶基因表达量的影响

doi:10.11843/j.issn.0366-6964.2014.07.013

Abstract

Abstract:

The aims of this study were to determine the variation of milk fat synthesis and expression of lipid anabolism related genes in liver tissue triggered by feeding a large amount of finely ground wheat inducing subacute ruminal acidosis (SARA)；and evaluate the ability of pelleted beet pulp (BP) as a substitute for ground corn to alleviate SARA.Eight mid-lactation Holstein cows were fed four diets during four successive 17-day periods：1)control period：total mixed ration (TMR) containing 0% finely ground wheat (FGW) (W0)；2) transition period：TMR containing 10% FGW (W10)；3)SARA induction period:TMR containing 20% FGW (W20)；and 4) SARA regulation period：TMR containing 10% BP as a replacement for 10% ground corn (BP10) on the basis of W20.The result shown that，compared to W0，the SARA induction protocol reduced (P<0.01) the mean ruminal pH from 6.37 to 5.94，the minimum ruminal pH decreased (P<0.01) from 5.99 to 5.41 from control period to SARA induction period，and the time of pH＜5.6 was more than 180 min•d^-1，SARA was induced.Mean ruminal pH increased from 5.94 to 6.05，and minimum pH increased from 5.41 to 5.63，when BP was substituted for corn.Dry matter intake and milk yield were not affected by the dietary treatments，however，milk fat percentage and yield were reduced (P<0.01) in the W20 and BP10 treatments than that in the W0 treatment.Cows fed the W20 diet had a lower (P<0.01) plasma concentration of triglyceride，and a higher (P<0.01) plasma concentration of glucose and insulin than cows fed the W0 diet.Liver tissue relative expression of acetyl-CoA carboxylase alpha (ACACA) (P=0.03)，FA synthase (FASN) (P=0.05)，sterol-response element binding protein 1 (SREBF1) (P<0.01) was increased，but there were no significant differences among the W10，W20 and BP10 diets.The results indicate that the SARA could decrease of milk fat and increase the relative expression of key lipid synthesis genes in liver tissue.The partly substitution of pelleted BP for ground corn in a high-concentrate diet could reduce the risk of SARA in dairy cows.

CLC Number:

GUO Yong-qing，ZOU Yang，XU Xiao-feng，CAO Zhi-jun，LI Sheng-li. Effect of Finely Ground Wheat Induced Subacute Ruminal Acidosis on Milk Fat Synthesis and Expression of Key Lipogenic Enzyme Genes in Liver Tissue in Dairy Cows[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1120-1128.

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Effect of Finely Ground Wheat Induced Subacute Ruminal Acidosis on Milk Fat Synthesis and Expression of Key Lipogenic Enzyme Genes in Liver Tissue in Dairy Cows

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